Tb^(3+)敏化化学发光法测定氟罗沙星

Determination of Fleroxacin by Tb^(3+) Sensitized Chemiluminescence

利用Tb3+ 能大大增强Ce H2 SO3 FLX的化学发光 ,建立了一种简单、快速、高灵敏度、高选择性的测定氟罗沙星的新方法 ,氟罗沙星浓度在 2 .0× 1 0 - 9～ 8.0× 1 0 - 8mol L范围内时 ,发光强度与氟罗沙星浓度呈良好的线性关系 ,方法的检出限为 8.3× 1 0 - 1 0 mol L ,对 2 .0× 1 0 - 8mol L的氟罗沙星平行测定 1 1次 ,相对标准偏差为 1 .2 %。并用该方法测定了沃尔得及天方片剂中氟罗沙星的含量 ,不经任何预处理 ,直接测定了尿样中氟罗沙星的含量及回收率 ,结果令人满意。

Based on the chemiluminescence reaction of Ce(IV)-H2SO3-fleroxacin (FLX) can be sensitized by Tb3+, a simple, rapid, high sensitive and high selective chemiluminescence method for the determination of FLX was developed. In the concentration range of 2.0 x 10(-9) similar to 8.0 x 10(-8) mol/L, the relative chemiluminescence intensity was linear with the concentration of FIX with a detection limit of 8.3 x 10(-10) mol/L. The relative standard deviation for the determination of 2.0 x 10(-8) mol/L FLX was 1.2% (n=11). When applied to the analysis of Woerde and Topfond tablets, the method was relatively free from the interference of common excipients. Without any pretreatment the content of FLX in human urine was determined and the result was satisfied.