群体反应性抗体与HLA分型技术在肾移植上的联合应用

Combined application of panel reactive antibody and HLA typing technique in kidney transplantation

目的 总结群体反应性抗体 (PRA)与HLA分型在肾移植联合应用上的意义。方法 PRA应用ELISA方法 ,HLA Ⅰ、ⅡAg应用单克隆抗体分型技术 ,对 2 0例肾移植病人手术前的PRA和HLA分型结果进行分析。结果 受者PRA 2 0 %以下者 12例 ,其余 8例为 2 0 %～ 5 0 %。供受者HLA A、B、DR完全符合交叉反应组 (CREG)为 13例。其中A、B、DR分别有一相同抗原的为 6例。术后 11例发生急性排斥 ,经冲击治疗 9例逆转 ,2例肾脏摘除 (1例PRA为 2 0 % ,1例PRA为 40 % )。 18例肾功能恢复。结论 PRA与HLA配型联合应用 。

Objective To summarize the significance of combined application of panel reactive antibody (PRA) and HLA typing technique in kidney transplantation.Methods ELISA was employed in PRA,and moncolonal antibody in HLA Ⅰ & Ⅱ typing.The preoperative results of PRA & HLA typing from 20 patients who will receive kidney transplantation were analyzed.Results Among the 20 recipients,PRA was less than 20% in 12,and 20%～50% in the other 8. HLA A,B,DR from the doners and recipients were completely matched with cross reactive group(CREG) in 13,including A,B and DR was synchronally present in 6.Acute rejection occurred in 11 postoperative patients,in which 9 were reversed by impulsive therapy and 2 underwent renal resection (PRA 20% in 1 and 40% in the other).Renal function recovered in 18.Conclusion The combined application of PRA and HLA typing technique can promote effects of renal transplantation.