HBe抗体阳性的乙型肝炎活动与静息HBV X基因变异的对比研究

ANALYSIS OF X GENE MUTATIONS IN HEPATITIS B VIRUS GENOME POSITIVE FOR ANTI-HBE BETWEEN ASYMPTOMATIC CARRIER AND PATIENTS WITH CHRONIC ACTIVE HEPATITIS

为研究乙型肝炎病毒 (HBV)X基因变异是否与e抗体阳性的HBV感染的不同临床现象相关 ,对 14例e抗体阳性的无症状携带 (AsC)及 14例e抗体阳性的慢性活动性肝炎 (CAH)病人的血清HBVX基因进行了克隆、测序研究。结果显示 ,14例AsC中 ,12例 (85 .7% )X基因上出现核苷酸插入、缺失、点突变 ,导致X基因编码的框移而提前终止 ,或提前出现终止密码子 ,从而使X蛋白被截短 ;而 14例CAH均编码 15 4个氨基酸的完整的X蛋白 ,没有出现上述X基因的变异 (P <0 .0 1)。本研究表明 。

To elucidate whether the mutations in X region of hepatitis B virus (HBV) might be responsible for the different clinical profiles in cases positive for antibody to hepatitis B e antigen. The nucleotide sequences of X gene regions in serum HBV were examined in 14 asymptomatic carriers (AsC) and 14 chronic active hepatitis (CAH) patients with antibody to hepatitis e antigen. The results showed that 12 of 14 AsCs (85.7%) had insertions, deletions or point mutations in nucleotide sequence of X region resulting in truncation of the X protein by creating frame shift mutation or a new stop codon, whereas no patient with CAH had those X gene mutations( P <0.01). It is concluded that in anti HBe positive HBV carriers, mutations resulting in the truncation of the X protein were the basis of the difference in clinical profiles, and were closely associated with a subsequent decrease in hepatitis activity.