哮喘患儿血浆内皮素、降钙素基因相关肽和一氧化氮的测定及临床意义

DETECTION AND CLINICAL SIGNIFICANCE OF ENDOTHELIN, CALCITONIN GENE-RELATED PEPTIDE AND NITRIC OXIDE IN PLASMA FROM CHILD PATIENTS WITH ASTHMA

目的 研究内皮素 (ET)、降钙素基因相关肽 (CGRP)和一氧化氮 (NO)在哮喘患儿的发病作用及临床意义。方法 对 2 2例重度哮喘发作患儿治疗前后用放射免疫法测定血浆ET、CGRP含量 ,用比色法测定NO含量 ,并与 2 0例健康儿童作对照分析。结果 哮喘急性发作期血浆ET、NO水平均高于缓解期及正常对照组 ,P <0 .0 1 ,哮喘缓解期ET含量仍高于正常对照组 ,P <0 .0 1 ,CGRP在急性发作期、缓解期与正常对照组差异无显著性 ,P >0 .0 5。结论 ET、NO参与哮喘发病过程 。

Objective To determine the significance of endothelin (ET), calcitonin gene-related peptide (CGRP) and nitric oxide (NO) for children suffering from asthma attacking. Methods Before and after the treatment, ET and CGRP were detected through radioimmunoassay and NO determined through colorimetry in 22 child patients suffering from severe asthma attacking. Comparison was performed with 20 healthy children. Results The plasma levels of ET and NO in the attacking stage were higher than those in the remission stage and also higher than those in the control group (P<0.01). The plasma level of ET in the remission stage was still higher than that in the control group (P<0.01). the level of CGRP showed no significant difference in the attacking stage, remission stage and the control group (P>0.05). Conclusion ET and NO had participated in the process of asthma. The rise of the levels of ET and No was important as a laboratory proof to the acute episode of asthma.