硒对汞致雏鸡肝功能损伤的拮抗作用

The Antagonistic Effects of Selenite on the Damage of Liver Function Initiated Mercury

选用健康伊沙褐雏鸡 2 5 0只 ,分为对照组、汞中毒组、3个水平的硒保护组 ,研究汞对血清中门冬氨酸氨基转移酶 (AST)、丙氨酸氨基转移酶 (ALT)、碱性磷酸酶 (ALP)、肝组织中谷胱甘肽过氧化物酶 (GSH Px)活性和丙二醛 (MDA)含量的影响以及硒与汞之间的相互作用效应。试验结果表明 ,汞可使血清中AST、ALT、ALP活性显著升高 (P <0 .0 1) ,使肝组织中GSH Px活性降低 (P <0 .0 1) ,引发肝组织脂质过氧化 ,致使肝组织中MDA含量显著增高 (P <0 .0 1) ,且有时间 效应关系 ;硒可使血清中AST、ALT、ALP活性显著降低 (P <0 .0 1) ,使肝组织中GSH Px活性显著升高 (P <0 .0 1) ,阻止肝组织脂质过氧化 ,致使肝组织中MDA含量显著降低 (P <0 .0 1) ,且有时间 效应关系。从而说明汞中毒损伤肝功能 ;硒对汞致肝功能的损伤具有拮抗作用。

In attempt to study the effects of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline phosphatase (ALP) activity in the serum, GSH Px activity and MDA content in the liver tissue from mercury, and interaction between selenite and mercury, 250 one day old chickens were randomly divided into five groups (1 mercury posioning group, 3 groups with diferent level of selenite protecting from mercury poisoning, and 1 control group). The results showed that the Mercury could marked improve AST, ALP activity in the serum (P<0.01), reduce the activity of GSH Px in the liver tissue (P<0.01) and it induce lipid peroxidation to improve the MDA content in the liver tissue (P<0.01) in a time effect rule; Selenite could marked reduce AST, ALT, ALP activity in the serum (P<0.01), improve the activity of GSH Px in the liver tissue (P<0.01) and it prevent lipid peroxidation to reduce the MDA content in the liver tissue (P<0.01) in a time effect rule. It can be conclude that mercury could damage liver functon and there was an antagonistic effects of selenite on the damage of liver function initiated mercury.