黄瓜花原基分化前后子叶的生理生化变化

Changes of physiology and biochemistry before and after differentiation of floral primordia in cotyledons of cucumber seedlings.

通过对黄瓜 1～ 7d苗龄子叶的生理生化变化研究表明 ,子叶中淀粉含量呈逐渐下降趋势 ,1～ 6d苗龄的可溶性糖含量快速上升 ,随后急剧下降 .POD活性和可溶性蛋白含量在苗龄 1～ 5 d也快速上升 ,随后也急剧下降 .苗龄 6、7d时的蛋白带比之苗龄 5 d以前少一条分子量较小的带 .子叶细胞的DNA含量基本保持恒定 ,但 RNA含量和 RNA/ DNA比值在苗龄 5 d前很快上升 ,随后急剧下降 .这些结果暗示子叶为黄瓜幼苗花原基分化提供了所需的碳架和氨基酸等 .

The investigations on physiology and biochemistry in cotyledons of 1 7 d cucumber seedlings showed that the starch contents in cotyledons decreased gradually and the soluble sugar contents in 1 6 d cucumber seedlings increased quickly then sharply declined, the activity of POD and the contents of soluble proteins in 1 5 d cucumber seedlings increased quickly then sharply declined too, a low molecular weight polypeptide was disappeared in cotyledons of 6, 7 d cucumber seedlings. The contents of DNA was invariable but the contents of RNA and RNA/DNA increased quickly in 1 5 d cucumber seedlings then sharply declined. It was considered that the carbon frame and the amino acids were needed for the differentiation of floral buds in cucumber seedlings and the physiology and biochemistry changes of cotyledons in the developmental stage was correlated to the differentiation of floral buds in cucumber seedlings.