山东省HCV分离株C区及NS5区核苷酸序列分析及其基因分型

Analysis of the nucleotide sequence for C and NS5 regions and the genotype of HCV isolate in Shandong Province

目的 研究山东省丙型肝炎病毒 (HCV)流行株的基因型别。方法 用反转录套式聚合酶链反应 (PCR)方法分别扩增山东省HCV流行株C区 (4 32bp)NS5区 (319bp)的两个基因片段 ,将其克隆入T载体上并自动测序 ,进行同源性分析及基因型别鉴定。结果 4株HCVC区基因片段有 3株为 1b基因亚型 ,1株为 2a基因亚型 ,10株NS5区的基因片段分析均为 1b基因亚型 ,并且与GenBank中多个 1b亚型代表株核苷酸序列同源性达 90 %以上。结论 山东省HCV流行株以 1b亚型为主 ,兼有 2a亚型。同一亚型中也有较大的变异 ,NS5区 1b亚型中基因离散率可达 5 %以上。

Objective To study the genotype of HCV epidemic strains in Shandong Province.Methods HCV fragments of C(432 bp)and NS5(319 bp) regions were amplified by RT nest PCR.The fragments were cloned to T vector and sequenced by dideoxynucleotide chain termination reaction.Results Three of all 4 strains in C region were 1b genotye,and 1 was 2a.All of 10 strains of NS5 region were 1b genotype.No other genotype was found in this region.The homology of nucleotide sequence was over 90% compared with that of more than 50 strains in GenBank.Conclusion The major genotype of HCV epidemic strains in Shandong Province is 1b.The 2a genotype is also found in this area.