卢龙县1998年婴幼儿腹泻轮状病毒血清型调查

The predomination of rotavirus G3 serotype among children with diarrhea in Lulong County in 1998

目的 对河北省卢龙县农村人口中儿童轮状病毒腹泻流行情况进行调查。方法 采用病毒核酸电泳和酶免疫方法对卢龙县 1998年轮状病毒腹泻发病季节采集的非菌性腹泻粪便标本进行轮状病毒检测。采用酶免疫和RT PCR方法对轮状病毒阳性标本进行血清型调查。结果 12 0份婴幼儿腹泻样本轮状病毒阳性率为 39 2 % ,病毒电泳型全为长型 ,血清分型发现G3型为流行优势株 ,占 61 7% ,其次为G1型占 36 2 % ,G4型占 6 4% ,轮状病毒腹泻病人最小年龄为 2月龄 ,最大为 2岁 ,男女之比为 1∶1 47。结论 卢龙县 1998年轮状病毒腹泻流行规律与全国情况基本一致 ,但流行毒株以G3型为主 。

Objective To investigate the epidemiology of rotavirus diarrhea among children in rural population.Methods 120 stool specimens collected from children with diarrhea during the winter of 1998 in Lulong County, Hebei Province were examined for rotavirus by PAGE and ELISA and for serotype by ELISA and PCR.Results Rotavirus was detected in children aged between 2\|24 months. The positive rate of rotavirus was 39.2% and the ratio of male to female was 1∶1.47. All rotavirus RNA profiles were long pattern. The distribution of rotavirus serotypes was type 3(61.7%), followed by type 1(36.2%), and type 4(6.4%).Conclusion The epidemiology of rotavirus diarrhea during 1998 rotavirus season in Lulong County was similar to that of other regions in China, but rotavirus serotype G3 was a prevalent strain in Lulong County, which was uncommon and different from the predominant G1 in other places throughout China.