一氧化氮合酶阳性神经纤维在小鼠心脏的分布

Distribution of nitric oxide synthase-contenting nerveous fibers in mouse heart

目的 探讨小鼠心脏一氧化氮合酶 (NOS)阳性神经纤维分布。方法 采用还原型尼可酰胺腺嘌呤二核苷酸磷酸硫辛酰胺脱氢酶 (NADPH -d)技术分析小鼠心脏的NOS神经纤维的结构和分布。结果 心脏各部均检出NOS神经纤维 ,多与肌纤维长轴平行走向。NOS阳性神经纤维心房最丰富 ,房室结次之 ,左、右心室最少。心房及房室结的NOS阳性纤维呈串珠状膨大 ,心室的常为丝状 ,膨体极少。结论 NOS阳性纤维在心脏各部的分布和形态均有差异 ,一氧化氮 (NO)可能作为神经递质和

Objective To observe the distribution of nitric oxide synthase (NOS)-contenting nerve fiber in mouse heart.Method Using reduced nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH-d) enzyme histochemical technique(staining),the distribution and structure of NOS-contenting nerve fibers in heart of mice were observed. Resurts The NOS-contenting nerve fibres were detected in all chambers of mouse heart,and majority of the fibers ran parallel with myocardial fibers. They were releatively abundant in the right and left atrial myocardium. There were even less numerous fibers in the atrioventricular node and ventricular muscle.The NOS-contenting fibers in the atrium as well as atrioventricular node were bead-liked bulgy in shape,and in the ventricular fiber,this bulgy were seldom and were filiform.Conclusion There are difference in pattern and dispersing of the NOS-contenting fibers in different charmber of mouse heart. The nitric oxide may play a role as a neurotransmitter and/or neuromodulator in the medulation and contral of the cardiovescular activities.