佐剂FCA与Quil A对rGST-Sj32的免疫增强作用的比较

COMPARISON BETWEEN FCA AND QUIL A IN IMMUNOPOTENTIATION OF RECOMBINANT FUSION PROTEIN GST-Sj32 VACCINATION IN MICE

目的 比较弗氏完全佐剂 (FCA)与皂素的纯化物 Quil A的免疫增强作用 ,进一步提高重组抗原 r GST- Sj32的免疫效果。 方法 r GST- Sj32 +FCA或 Quil A免疫 NIH小鼠 3次 ,EL ISA法检测抗体水平 ,用减虫率及减卵率表示保护效果。 结果 与 PBS对照组比较 ,r GST- Sj32 +FCA免疫小鼠减虫率为 42 .9% ,每克肝卵 (L EPG)减少率 5 1.0 % ,每雌肝卵 (L EPF)减少率为 2 3.9% ,抗体滴度达 1∶ 2 5 6 0 0。r GST- Sj32 +Quil A免疫小鼠减虫率为 46 .0 % ,L EPG减少率39.4% ,L EPF减少率为 8.5 % ,抗体滴度达 1∶ 5 12 0 0。 结论 FCA和 Quil A均可增强 r GST- Sj32免疫小鼠的抗感染保护性免疫力 ;FCA亦可增强 r GST- Sj32诱导小鼠抗生殖免疫 ,Quil A无增强抗生殖免疫的作用。

Objective To compare the immunoenhancing effects between two adjuvants,Freunds complete adjuvant(FCA) and Quil A(purified saponin),in mice vaccinated by recombinant fusion protein SjGST-32(rGST-Sj32). Methods Mice were immunized three times with rGST-Sj32 plus FCA or Quil A . The worms and the liver eggs from the mice challenged with 40 cercariae of Schistosoma japonicum after 45 days were counted. Results As compared with PBS control group,the rGST-Sj32+FCA group and rGST-Sj32+Quil A group showed that the worm reduction rates was 42.9% and 46.0% respectively,the liver egg per gram(LEPG) reduction rate was 51.0% and 39.4% respectively, and the liver egg per female(LEPF)reduction rate as 23.9% and 8.5% respectively. And the specific antibody titers reached 1∶25 600 and 1∶51 200 respectively. Conclusion FCA or Quil A plus rGST-Sj32 could induce significant protective immunity in mice. However, the former induced significant anti-fecundity immunity, and the latter was not showing such an effect.