乙酸β-哌啶乙酯的灭螺作用与钙离子的关系

RELATIONSHIP BETWEEN ACTION OF ACETIC-β-(N-PIPERIDINE)-ESTER ON SNAIL-KILLING AND CALCIUM ION

目的 研究乙酸 β-哌啶乙酯 (Ape)的杀螺作用与 Ca2 +的关系。 方法 采用完全浸泡和离体钉螺足平滑肌的实验方法 ,研究 Ape杀螺效果及其对 Ca2 +的影响。 结果 0 .1mg/ L和 0 .5 mg/ L Ape均有杀螺作用 (P均 <0 .0 5 ) ,且呈浓度依赖性。不同浓度的 Ape对钉螺足平滑肌的收缩效应曲线呈双相性。低浓度时 (10 - 7～ 3× 10 - 6 mol/ L)可增强其收缩幅度 ,且能被钙通道阻滞剂维拉帕米所拮抗 ;高浓度时 (10 - 5～ 10 - 4 mol/ L)则能拮抗钙通道激动剂 Bay K86 44增强钉螺足平滑肌的收缩幅度。 结论 Ape具有一定的杀螺作用 ,其影响平滑肌收缩的结果表明 ,高浓度有阻 Ca2 +作用。

Objective To study the relationship between action of acetic-β-(N-piperidine)-ester (Ape) on snail-killing and calcium ion. Methods Immersing method of 'Final screen method in lab of snail-killing drugs' issued by WHO and contractive activities of isolated foot smooth muscles of oncomelania were used in the study. Results 72 hour death rate of oncomelania was 7.8% and 13.0 %(P<0.05) respectively at 0.1 mg/L and 0.5 mg/L concentration of Ape. Different concentration of Ape showed a biphasic concentration-effect. At 10 -7 ～3×10 -6 mol/L, it increased the contractility of oncomelania foot smooth muscle,while at 10 -5 ～10 -4 mol/L, it inhibited the contractility gradually. Ape 3×10 -5 mol/L obviously inhibited the contractive activities with Bay K 8644 reinforces in foot smooth muscles of oncomelania. 5×10 -6 mol/L of Verapamil obviously inhibited the contractive activities with 3×10 -6 mol/L of Ape reinforces in foot smooth muscles of oncomelania. Conclusion Ape has a certain effect on killing oncomelania, may be via inhibiting calcium ions inflow and flacciding smooth muscles of oncomelania.