大鼠急性胰腺炎胰腺细胞凋亡改变的实验研究

The experimental study of pancreatic acinar cell apoptosis in acute pancreatitis rats

目的 :了解急性胰腺炎 (AP)发病过程中胰腺腺泡细胞凋亡情况。方法 :将 48只SD大鼠随机分为两组 (胰腺炎组和假手术组 ) ,每组 2 4只。经十二直肠行胆胰管逆行加压注射 4%牛磺胆酸钠 ,诱导大鼠AP模型 ,于术后 3小时、6小时、12小时和 2 4小时分批处死动物 ,应用末端脱氧核苷酸转换酶 (TdT)介导的末端标记 (TUNEL)方法检测胰腺组织中的腺泡细胞凋亡。结果 :假手术组胰腺组织中存在极少量的凋亡细胞 ,数量相对恒定 ,与时间无关 ;胰腺炎组术后病变加重 ,胰腺凋亡细胞明显增多 ,3小时、6小时、12小时凋亡指数均明显高于假手术组 (P <0 .0 1) ,术后 2 4小时接近假手术组水平。结论 :急性胰腺炎胰腺存在腺泡细胞凋亡的改变 。

Objective:To explore pancreatic acina cell apoptosis in rats with acute pancreatitis (AP).Methods:48 rats were randomaly divided into two groups, AP group (n=24) and sham operation (SO) group (n=24). The model of AP was induced by retrograde injection of 4% sodium taurocholate into biliopancreatic duct in rats. At 3h,6h,14h and 12h, groups of rats (n=6) were sacrificed by cercial dislocation. A terminal deoxynucleotidyl transferase mediated dUTP biotion nick end labeling (TUNEL) method was used to detect apoptosis of pancreatic acinar cell and apoptosis index (AI) was evaluated.Results:Very few apoptoic acinar cells were found in SO rats. Extensive and time dependent pancreatic acinar cell apoptosis were found after AP was induced. The valuels of AI gradually rose, a maximal value presented at 6h and then declined rapidly, close to the SO level at 24h. AI of AP rats at 3h, 6h and 12h, compared with that of SO group, was significantly higher (P<0.01).Conclusion:The fact that apoptosis of pancreatic cell does exist and that pancreatic cell undergoes apoptosis maybe an important pathologic feature during AP.