胰腺腺泡细胞凋亡在大鼠急性胰腺炎病程中的作用及凋亡调控基因的表达

Role of apoptosis of acinar cell and expression of bax in rats with acute pancreatitis

目的 :探讨胰腺腺泡细胞凋亡及凋亡调控基因———Bax的表达情况及其与急性胰腺炎严重度的关系。方法 :在胰胆管共同通道内逆行注射不同浓度的脱氧胆酸钠 ,制备不同重症程度的急性胰腺炎模型。2 4只SD大鼠随机分为对照组 (假手术组 )、APⅠ组 (0 .75 %剂量组 )和APⅡ组 (1.5 %剂量组 )。分别于造模后6小时处死各组存活大鼠。用TUNEL法分析腺泡细胞凋亡情况 ,用免疫组化及WesternBlotting检测Bax的表达情况。结果 :APⅠ组大鼠较APⅡ组胰腺坏死减少、出血减轻 ,血清淀粉酶、IL 6和IL 8水平明显下降 ;病变程度轻的APⅠ组大鼠腺泡细胞凋亡指数比病变程度重的APⅡ组高 (6 .36± 2 .41vs 2 .34± 1.15 ,P <0 .0 5 ) ;正常腺泡细胞Bax的表达弱 ;产生急性胰腺炎时 ,腺泡细胞Bax的表达增强 ,病变程度轻的大鼠腺泡细胞Bax表达水平较病变程度重的强 ,与二者凋亡指数的差别相一致。结论 :产生急性胰腺炎时 ,腺泡细胞凋亡与其病情严重程度呈负相关 ,腺泡细胞凋亡在急性期是一种保护现象 ;Bax的表达水平与腺泡细胞凋亡指数呈正相关 。

Objective:To study the relationship between the pancreatic acinar cell apoptosis and the severity in rats with acute panreatitis.Methods:The different severity of acute pancreatitis model of SD rat was established by retrograde injection of different concentration of sodium deoxycholate into the common bile pancreatic duct. Rats (n=24) were divided at random into Control group, APⅠ group (0.75% dose group) and APⅡ group (1.5% group). The apoptosis of acinar cell in acute pancreatitisis was analysed by TUNEL,and the expression of Bax protein detected by immunohistochemical method and Western Blotting.Results:The apoptosis index was higher in APⅠ group than in APⅡ group (6.36±2.41 vs 2.34±1.15 in 6hr,P<0.05), which was in correspondence with the degree of Bax expression.Conclusion:The degree of apoptosis is inversely related to the severity of acute pancreatitis,which suggests that apoptosis may be a teleologically beneficial response to acinar cell injury in general and especially in acute pancreatitis. The expression of Bax is positively related to the apoptosis index of acinar cell and negatively related to the degree of acute pancreatitis.