胃癌P_(53)蛋白与增殖细胞核抗原表达及其临床意义

The expression of P_(53) protein of carcinoma of stomach and the proliferative cell nuclear antigen

应用ABC免疫组织化学方法 ,研究了 66例胃癌P53 蛋白的表达及其与细胞增殖活性、淋巴转移的关系。结果表明 :62 .2 %胃癌组织中存在P53 蛋白的异常表达。P53 蛋白阳性的胃癌其细胞增殖活性及淋巴结转移机率均较阴性者高 (P <0 .0 5 )。提示P53 基因的突变以及由此导致的细胞异常增殖不仅与胃癌的发生有关 ,而且在其淋巴结转移中也起重要作用。检测P53 蛋白表达对判断肿瘤的恶性程度有重要参考价值。

An ABC immunohistochemical method was used to study the relationship between the expression of P 53 protein and the cell proliferative activity. Results showed that 62.2% of the tissues of stomach of carcinoma had the abnormal expression of P 53 protein. The cell proliferative activity and the probability of lymph-node metastasis in carcinoma of stomach with positive P 53 protein were higher than those in the carcinoma of stomach with negative one (P<0.05). This study indicated that not only had the P 53 gene mutation and the abnormal cell proliferation correlation with the incidence of stomach of carcinoma, but also played an important role in the lymph-node metastasis. The detection of the expression of P 53 protein is of great reference value to the diagnosis of the tumor's malignant degree.