肿瘤放免治疗后免疫功能变化及其与预后关系

ALTERATION OF IMMUNOFUNCTION OF THE PATIENTS WITH CARCINOMA RECEIVING RADIOIMMUNOTHERAPY

目的 :通过检测 2 6例胃肠道恶性肿瘤患者接受放射免疫治疗 (放免治疗 )前、后LAK细胞活性及其白细胞介素 - 2受体 (IL - 2R)表达和T淋巴细胞亚群改变 ,以及研究这些变化与疗效的关系 ,以求揭示放免治疗对机体免疫功能的影响及放免治疗的抗肿瘤机理。方法 :LAK活性检测采用MTT法 ;白细胞介素 - 2受体(IL - 2R)表达应用免疫荧光法检测 ;用流式细胞仪检查T淋巴细胞亚群改变。结果 :放免治疗后 2周患者外周血CD3 + 、CD4 + 细胞增多 ,CD4 + /CD8+ 比值增高 ,LAK细胞活性增强 ,IL - 2R受体阳性LAK细胞数明显增多 ,CD8+ 细胞无明显改变 ;治疗后显效组外周血CD3 + 、CD4 + 细胞、CD4 + /CD8+ 比值、LAK细胞活性及IL -2R受体阳性LAK细胞数明显高于非显效组 ,而治疗前无明显差异。结论 :放免治疗可增强机体免疫功能。

Objective:To explore the alteration T lymphcyte subsets、LAK cells activities and IL-2R positive LAK cells in 26 patients with carcinoma undergoing radioimmunotherapy using anti-tumor monoclonal antibody CL 3 labelled with 131 Ⅰ( 131 Ⅰ-CL 3) local injection and to explore the correlation between the alteration of immunofunction and therapeutic effect.Methods:The determination of T lymphocyte subsets was performed with APAAP.The measurment of LAK cells activity was performed with MTT.Results:After the intralesional injection of 131 Ⅰ-CL 3,CD + 3,CD + 4 T lymphcytes and CD 4 +/CD 8 +ratio increased and LAK cells activity was enhanced.There were no alteration in CD + 8 cells.The enhancement of immunofunction of the patients having significant therapeutic effect was more remarkable than that of patients having unsignificant therapeutic effect.Conclusions:Radioimmunotherapy could enhance the immunofunction of patients with carcinoma.There was significant correlation between the enhancement of immunofunction and the therapeutic effect of the radioimmunotherapy.