人—猪链球菌感染性综合征的流行病学研究

The Epidemiological Study on Human Streptococcal Infectious Syndrome Caused by Infected Pigs

目的:研究人—猪链球菌感染暴发事件的病因、流行因素,为治疗、控制及预防提供科学依据。方法:观察26例原因不明的“急性感染性中毒性出血性休克综合征”,进行了个案调查和1∶4病例对照研究,以及分离培养病原、菌种鉴定等。用PCR指纹图技术分析人源与猪源株同源性。结果:此次江苏如皋市及相邻地区发生的人链球菌感染性综合征暴发疫情,是因当地猪链球菌病流行,人与病死猪直接接触引起,其相对危险度为40.89。从患者的血液和脑脊液分离到的链球菌,经生化、体脂肪酸分析、血清凝集等系统鉴定为猪链球Ⅱ型。经流行病学调查等证实,此起疾病为感染性疾病,人与人之间未见传染性。与接触病、死猪有关。随机引物PCR技术分析显示,人源与猪源菌株系同源。结论:本起疾病的病源体为猪链球茵Ⅱ型,感染来源为直接接触病、死猪。

Objective:To research the pathogeny and prevail factor of the manifestations of S. suis infective syndrome; to provide gists for treatment and control and prevent. Methods: 26 cases with the human streptococcal infective syndrome were identified. A 1:4 matched case-control study was carried out and the cause of disease pathogeny was severed and cultivated to identify fungus.The human born strains and the pig born strains were compared with RAPD technique. Results: The human case of streptococcal infective syndrome were found epidemiologically to be contracted by contacting with the infected pigs or infected pig corpses in Rugao and region borolering upon, with OR of 40.89.The strains of streptococcus isolated from the blood and cerebropinal fluid(CSF) of infective men were S. suis type 2 by biochemical tests and analysis of fatty acid of thalli and serum coagulate tests.The study of epidemiology proved that this disease wasn't infective among human.The strains of streptococcus from human and pigs were isogenous by RAPD techique. Conclusions:The case was caused by S. suis type 2 from contacting with the infected pigs or infected pig corpses.