依诺沙星的铽敏化化学发光法

TERBIUM SENSITIZED CHEMILUMINESCENCE OF ENOXACIN

目的 建立片剂及尿样中依诺沙星含量的铽敏化化学发光检测法。方法 用流动注射仪将Ce4+ ,H2 SO3,Tb3+ 和依诺沙星按一定顺序泵入流通池 ,用化学发光检测器测量体系的相对发光强度。结果 依诺沙星浓度在 2 0× 10 - 9- 6 0× 10 - 8mol·L- 1 相对发光强度与浓度呈良好的线性关系 ,检出限为 4 0× 10 - 1 0 mol·L- 1 。对浓度为4 0× 10 - 8mol·L- 1 的依诺沙星进行 11次的平行测定 ,RSD为 1 6 %。结论 为研究依诺沙星药动学提供了灵敏、简便、快速。

AIM To determine the enoxacin by means of terbium sensitized chemiluminescence in pharmaceutical preparations as well as urine samples. METHODS Through six way injection valve Ce 4+ , H 2SO 3, Tb 3+ and enoxacin (ENX) standard solutions were injected into the flow system in a certain order, the chemiluminescence signal was detected by weak luminescence analyzer. RESULTS The chemiluminescence intensity was linearly related to the concentration of ENX in the range of 2 0×10 -9 -6 0×10 -8 mol·L -1 with a detection limit of 4 0×10 -10 mol·L -1 . Relative standard deviation was less than 1 6% ( n =11). CONCLUSION It provides a sensitive rapid, simple and accuracy measurement of enoxacin for the pharmacokinetic studies.