系统性红斑狼疮病人环孢素A受体mRNA的表达

Cyclophilin mRNA Expression in Peripheral Mononuclear Cells from Systemic Lupus Erythematosus Patients

目的 探讨静止期、活动期系统性红斑狼疮病人 (SLE)外周血单个核细胞 (PBMC)环孢素A受体 (CyP)mRNA表达及糖皮质激素对其表达的影响 ,为临床应用环孢素A辅助治疗该病提供理论依据 .方法 采用逆转录PCR方法 ,经凝胶图像半定量分析 ,检测患者外周血单个核细胞CyPmRNA的表达 .结果 SLE病人外周血单个核细胞存在有CyPmRNA的表达 ,静止期较正常人差异无显著性 (p>0 .0 5 )意义 ,但在活动期CyPmRNA的表达较正常人和静止期病人明显降低 (p <0 .0 1) ,地塞米松处理后 ,活动期病人CyPmRNA的表达水平显著上升 (p <0 .0 1) .结论 SLE病人有CyPmRNA的表达 ,但活动期明显下降 。

Objective To investigat the cyclophilin(CyP) mRNA expression in peripheral mononuclear cells from systemic lupus erythematosus(SLE) patients and the influence of glucocotisteroid treatment ,so as to obtain some theoretic evidences for cyclosporin A(CsA)used as supplementary treatment in SLE. Methods Using reverse transcription polymerase chain reaction (RT-PCR) method,CyP mRNA was analyzed through gel image analysis. Results CyP mRNA was expressed in all groups ,and there was no significant statistical difference between static stage SLE group and normal control group(p>0.05).but it was lower in active stage group(p<0.01).Dexamethasone treatment could up-regulate CyP mRNA expression in active stage SLE group. Conclusions CyP mRNA is expressed in the peripheral mononuclear cells of SLE patients,but declines in active stage patients.Glucocotisteroid treatment can ameliorate the CyP mRNA expression of active stage patients.