小于胎龄新生幼鼠肝脏组织IGFs基因表达的变化

RESEARCH ON THE CHANGES OF HEPATIC IGFS GENE EXPRESSIONS IN SMALL FOR GESTATIONAL AGE RATS

目的 :在宫内发育迟缓 (IUGR)模型的基础上 ,测定小于胎龄 (SGA)新生幼鼠肝脏组织胰岛素样生长因子 -Ⅰ(IGF -Ⅰ )及其结合蛋白 (IGFBPs)的基因表达变化 ,以阐明它们在发病中的作用。方法 :子宫动脉结扎 (UAL)建立IUGR模型 ,用逆转录 (RT) -PCR分析SGA新生幼鼠肝脏组织IGF -Ⅰ、IGFBPsmRNA表达变化。结果 :轻度SGA肝脏组织IGF -ⅠmRNA水平无明显改变 ,IGFBP1 mRNA水平升高 ,重度SGA同时伴有IGF -ⅠmRNA水平下降 ,IGFBP1 、IGFBP2 mRNA水平上升。结论 :肝脏组织IGF系统基因表达异常可能是胎儿宫内生长发育迟缓的重要病因之一。

Objective:In order to elucidate the roles of insulin like growth factor-Ⅰ(IGF-Ⅰ) and IGF binding proteins (IGFBPs) gene expressions in the newborns small for gestational age(SGA),hepatic IGF-Ⅰand IGFBPs mRNA expressions on the basis of the model with intrauterine growth retardation (IUGR) were measured .Methods:We examined the impact of IUGR on uterine arteries ligation (UAL).RT-PCR analysis of IGF-Ⅰand IGFBPs mRNA contents in livers of SGA rats.Results:In mild SGA group,hepatic IGF-ⅠmRNA content has no change and IGFBP 1 and IGFBP 2 mRNA contents were slightly increased .In severe SGA group, IGF-ⅠmRNA content was deeply decreased, IGFBP 1 and IGFBP 2 mRNA contents were significantly increased.Conclusions:The changes of hepatic IGF-Ⅰand IGFBPs mRNA expressions would probably contribute to the developments of IUGR.