摘要
本文综述了细菌人工染色体 (BacterialArtificialChromsomes ,BAC)的构建、物理图谱制作方法及其靶位精细操作策略的研究进展。同时 。
This paper reviewed the progress on the development of Becterial Artificial Chromosomes (BAC),construction approaches of physical mapping and strategies of performing targeted alterations such as site\|directed mutagenesis,deletions and replacement in BAC.Furthermore,the application of BAC transgenes in studying of the gene function,the phenotypic expression of an entire gene,or even distant regulatory sequences in their native sequence environment is also introduced.
出处
《生物工程进展》
CSCD
2001年第4期3-7,共5页
Progress in Biotechnology
基金
淡水生态与生物技术国家重点实验室 (FEBL)基金资助
