HeLa细胞中hR24L基因的表达、DNA损伤修复、G_2/M阻滞与电离辐射之间的关系(简报)

RELATIONSHIP OF hR24L GENE EXPRESSION, DNA DAMAGE REPAIR AND G2/M PHASE CELL CYCLE ARREST WITH ION RADIATION

DNA是生命活动中最重要的遗传物质,保持其分子结构的完整性对于细胞至关重要,因此研究DNA损伤修复是生命科学的重要课题之一.基因组比较简单,易于操作的单细胞真核生物酵母遂成为研究DNA损伤修复的重要材料.对紫外线或电离辐射敏感的酵母突变株称为rad突变株.酵母细胞的基因组中有近30个遗传位点与辐射抗性有关.根据单突变和双突变的敏感特征所得出的上位关系可将其分为3个上位显性组[1,2]:RAD3组,该组成员参与核苷酸的切除修复,其突变株对紫外线敏感;RAD6组,参与复制后修复,其突变株对化学诱变剂敏感;RAD52组,参与重组修复(包括链断裂修复),其突变株对电离辐射敏感.RAD24基因属于RAD3组,兼有其他组的某些特性.

The KR24L gene ORF was cloned from the total RNA of HeLa cells by RT - PCR method. There is no mutation of the hR24L gene in HeLa cells. Ion radiation significantly increased the expression of hR24L gene, and the sense hR24L enhanced and accelerated the cell cycle arrest at G2/M phase in HeLa cells. Besides, it should seem that the overexpression of hR24L gene could enhance the repair ability of DNA damage induced by ion radiation, and vice versa.