稳定表达黄嘌呤氧化还原酶的人类神经外胚层瘤PFSK细胞株的建立(简报)

ESTABLISHMENT OF HUMAN NEUROECTODERMAL TUMOR CELL LINE (PFSK)WITH STABLE EXPRESSION OF XANTHINE OXIDOREDUCTASE

黄嘌呤氧化还原酶(Xanthine Oxidoreductase,XOR)参与嘌呤类物质代谢,是嘌呤代谢的关键酶.在哺乳动物中,XOR以两种可互相转换的形式存在,即黄嘌呤脱氢酶(XDH)和黄嘌呤氧化酶(XO)[1,2].同时,由于XOR催化反应的副产物是氧自由基,因此XOR参与氧自由基产生的作用也日益受到重视.大量动物实验模型已经表明在氧化性组织损伤过程如脑缺血/再灌注等病理情况下,XOR活性增强导致的氧自由基积聚是造成组织损伤的直接原因之一[3-6].但是,人类XOR的研究却由于正常人类XOR活性和基因表达水平较低(仅为啮齿类动物的1%[7])等原因受到了阻碍,直到近期才有一些进展[8,9].而对于XOR在人类生理及病理过程中除参与嘌呤代谢外的其他作用则尚未确定.

Xanthine Oxidoreductase (XOR) is the key enzyme in purine metabolism and also produces oxygen free radicals. As a continuation of our previous work, in this study, we constructed a retrovirus expression vector (PLNCX2-XOR) containing full length of XOR cDNA. Retrovirus was produced by the virus package cell line PT - 67 following the transfection of PT-67 with pLNCX2- XOR and used to infect the human primitive neuroecto-dermal tumor cell line (PFSK) . Infected PFSK cells were selected by G418 to establish cell line with stable expression of XOR. The expression of XOR in the cell line we established was confirmed by RT-PCR, Immunocytoche-mistry and XOR activity assay.