沙田柚系列遗传关系的RAPD标记研究

Analysis of genetic relationships among Shatianyou Pomelo lines by RAPD markers

采用 RAPD标记技术分析了沙田柚系列 1 2个样品的遗传关系。利用经筛选具多态性的 1 4个 1 0碱基随机引物对 1 2个样品进行 DNA随机扩增 ,获得清晰可重复的位点 99个 ,其中多态性位点 5 8个 ,占 5 8.89% ;在部分样品中检出了特异性 RAPD标记 1 9个 ,占 1 9.1 9% ;通过样品间遗传相似性系数比较与 UPGMA聚类分析 ,并根据历史事实可知 ,广西沙田柚、梅州金柚为沙田柚的无性繁殖系 ,软枝系沙田柚、沙田柚早熟单株为沙田柚的芽变后代 ,梅花早柚、菊花心沙田柚、冬瓜圈沙田柚、段氏柚、垫江沙田柚、古老钱沙田柚为沙田柚的实生变异品系。

Genetic relationships among 12 accessions of Shatianyou(Citrus grandis Osbeck cv.)Pomelo lines were analyzed by RAPD technique. Fourteen screened 10 bases primers were used in the study.They produced 99 clear and repeatable RAPD bands, in which 58 were polymorphic, accounting for 58 59%, and 19 were cultivar specific RAPD markers, accounting for 19 19% .The results of genetic similarity analysis and UPGMA clustering showed that Guangxi Shatianyou(cv.)and Meizhou Jinyou(cv.)were the clones of Shatianyou(Citrus grandis Osbeck cv.); Ruanzhixi Shatianyou(cv.)and Shatianyou precocity individual were the bud mutation of Shatianyou(Citrus grandis Osbeck cv.); The others were selected from seeds of Shatianyou(Citrus grandis Osbeck cv.)originating from hybridization.