AFLP在芒果品种鉴定中的应用

Application of AFLP in the cultivar identification of mango

利用 AFLP分子标记技术对 1 6个芒果 (Mangif era indica L.)品种以及 7个芒果砧木材料进行了初步研究 ,6对 AFLP选择性引物共产生 2 0 4条清晰谱带。同时利用品种间共有谱带率 (Band-sharing)对某些品种间的遗传关系进行了分析。 1 6个栽培品种间的平均共有谱带率为 83 % ,变异幅度为 70 %～ 94% ,7个砧木间的平均共有谱带率则为 80 % ,变异幅度为 5 3 %～ 96%。

AFLP was first used to study 16 mango cultivars and 7 mango rootstocks, and 6 AFLP selective primer pairs devoted 204 clear bands. The genetic relationships of some mango cultivar pairs and those of some mango rootstock pairs were studied by their Band Sharing. The Band Sharing average of the mango cultivars was 83%, and their variant range was 70%～94%. For the rootstocks, the Band Sharing average and the variant range were 80% and 53%～96%, respectively.