DNA指纹图谱鉴别双歧杆菌的研究

DNA FINGERPRINTING CHARACTERIZATION OF Bifidobacteria

采用RAPD技术选用 10条引物对 7种 9株双歧杆菌基因组DNA进行PCR扩增 ,根据在优化条件下所得DNA指纹图谱分析了双歧杆菌菌株的遗传多样性 ,并构建了相似性指数矩阵和树状图。结果表明 ,不同序列的随机引物可扩增不同形式的RAPD图谱 ,但并非所有图谱都具有分类学意义 ,其中引物S2 56对双歧杆菌种及同种不同菌株均具有良好的区分能力 ,由该引物扩增的RAPD图谱计算出的相对性指数矩阵以及由此构建的聚类树状图均能正确地反映出双歧杆菌的系统发育关系 。

primers were chosen randomly to PCR amplify genomic DNA from 9 strains belonging to 7 species of Bifidobacteria after analyzing DNA fingerprinting patterns and calculating their similarity indices (SI). It was found that different species or strains of Bifidobacteria showed different DNA finger printing when using S\-\{256\} primers. The possibility of using RPAD patterns as molecular markers for Bifidobacteria strains is discussed.\;