巨大芽孢杆菌在维生素C二步发酵中的作用

THE ROLE OF Bacillus megaterium IN TWO-STEP VITAMIN C FERMENTATION

巨大芽孢杆菌培养时期不同其上清液对促进氧化葡萄糖酸杆菌生长和产酸能力不同 ,在稳定期及衰亡初期显著促进氧化葡萄糖酸杆菌生长与产酸。pH、温度可改变上清液对氧化葡萄糖酸杆菌生长与产酸的影响。上清液中含活性物质具有蛋白质的部分性质 ,对酸、碱。

Culture supernatant of Bacillus megaterium (B. m.) at different period has different promotion on the growth of Gluconobacter oxydans (G. o.) and acid production ability. B. m. at stable stage and at early declining stage remarkably promote G. o. to grow and to produce acid. Temperature, time, amount, and pH, however, could change the effect of the supernatant on acid production of G. o. The active material of supernatant has some characteristics of protein, such as sensitivity to extreme pH and high temperature.\;