木瓜中齐墩果酸测定方法的研究

STUDIED ON CORLORIMETRIC DETERMINATION OF OLEANOLIC ACID IN CHINESE QUINCE

研究了木瓜中齐墩果酸的比色测定方法 ,试验证明 :用香草醛 高氯酸为显色剂的最佳测定条件为 :加入待测试样后 ,加热挥去溶剂 ,再加入 0 2ml新制的 5 %香草醛冰醋酸液及 0 8ml高氯酸试剂 ,在 70℃恒温水浴加热 15min ,冷却加入 4ml乙酸乙酯 ,摇匀 ,在λ =5 6 0nm处测定。该法操作简单 。

Colorimetric determination of loeanolic acid in Chinese quince was investigated in the present work.Experimental results revealed that the optimal condition was determined when vanillin perchloric acid served as a corloring reagent.Added samples were warmed up for drying up. 0.2 ml of freshly prepared 5% vanillin in acetic solution and 0.8 ml of perchloric acid were then added.The mixture was warmed up in a water bath at 70 ℃ for 15 min.After cooling down,4 ml of ethyle acetate was added in and well shaken,and samples were measured colorimetrically at λ=560 nm.This colormetric assay is simple,sensitive and relatively precise.