同义密码子用语的位置依赖

POSITION DEPENDENCE OF SYNAMOUS CODON USAGE

研究了大肠杆菌编码区不同位置上的同义密码子用语 ,发现许多氨基酸的密码子用语在转译起始区有显著的变化 ,仅有少数氨基酸在转译终止区有较弱的变化。由于密码子用语与基因表达关系密切 ,这些结果与实验发现的编码区5'端密码子用语对表达的重要性是一致的。更进一步的结果还暗示了哪些密码子在特定位置的使用可能会影响基因表达。

Synonymous codon usage of E.coli at different positions along the coding sequence was investigated. Many amino acids show significant variation in codon usage in the translational initiation region and only a few amino acids show weak variation in the translational termination region. Since codon usage is closely related to gene expression, the results coincide with the experimental findings that codon usage at the 5' end of the coding sequence is very important for gene expression. Furthermore, the results indicate that which codons at specific positions may influence gene expression.