新疆维族apoE基因多态性与寿命和血脂关系的分析

Relationship between apolipoprotein Egene polymorphism and longevity , lipids in Uygru Nationality

目的 探讨载脂蛋白 E(apo E)基因多态性与人类寿命的关系。方法 采用 PCR- RFLP技术检测了 1 60名维族 apo E基因型 ,其中长寿老人 35名、男性心肌梗死病人 54名、青年男性 71名 ,就 apo E基因频率分布及血脂的关系进行了比较分析。结果 维族长寿老人 ε4等位基因的频率最低 (0 .0 57) ,普通青年男性居中 (0 .1 2 7) ,心肌梗死病人最高 (0 .2 1 3) (P<0 .0 1 )。冠心病病人中 ε4携带者心肌梗死首次发病年龄平均为 51 .3岁 ,而非携带者为 58.3岁 ,两组比较 P<0 .0 5。 apo E基因与血脂的关系在长寿老人和青年对照组表现为ε4等位基因携带者血清总胆固醇和低密度脂蛋白胆固醇最高 (P<0 .0 5) ,但冠心病病人中无此差异。结论 ε4等位基因携带者的平均寿命低于其他等位基因携带者 ,其机制还有待于从不同方面进一步研究。

Objective To probe the re la tion between apoE alleles and life-span.Methods ApoE gen otypes were determine d by PCR-RFLP in 160 Uygru cases(35 cases age>90, 71 males age 20～35 and 54 ma les with myocardial infarction) and relationship between the frequencies of apoE gene and lipid were also analyzed.Results Significan t variations were shown in the ε4 allele ,the lowest in the age d (0.057) and the highest in patients of MI. The average age of first MI attack was significantly earlier in theε4 allele carriers than the not carriers, 51.3 and 58.3 years respectively (P<0.05). A correlation was shown betweenε4 al lele and high level total cholesterol and LDL-cholesterol in the aged and youn g groups, but not in MI group.Conclusions ApoE genotype may have an impact on human longevity, but the mechanism remains to be investig ated.