抗酒石酸酸性磷酸酶测定及在骨质疏松症诊断中的应用

Determination of serum tartrate-resistant acid phosphatase and its clinical application in diagnosis of osteoporosis

目的 建立抗酒石酸酸性磷酸酶测定方法及在原发性骨质疏松症诊断中的临床应用。方法 以 1 -萘酚磷酸为基质 ,测定肝素抗凝血抗酒石酸 ,氟化物抑制酸性磷酸酶 ,并以双能 X线骨密度仪测定其腰椎 2～ 4的骨密度。结果 抗酒石酸酸性磷酸酶测定方法在本地区老年妇女的参考值为 2 .9～ 5.3U/ L,该方法的平均批内和批间变异分别为 2 .7%和 5.0 %。骨质疏松症该酶活性明显高于健康对照组 (P<0 .0 0 1 ) ,并与骨密度测定呈显著的相关性 (r=0 .82 )。结论 抗酒石酸酸性磷酸酶测定方法简便、快速 ,是早期原发性骨质疏松症的筛选、诊断及观察疗效的敏感指标。

Objective To establish a me thod for determination of tartrate-resistant acid phosphatase (TrACP) and evalu ate its application in diagnosis of osteoporosis.Methods Th e activities of tartrate-resistant and fluoride-sensitive acid phosphatase wer e measured by using 1-naphthol phosphate as substrate and the bone density was examined by dual energy X-ray bone mineral densitometer. Results The reference range of this method was 2.9～5.3 U/L in the native old women. The CV (coefficient of variation) of this method was 2.7% (within-run) a nd 5.0% (between-run) respectively. The activity of TrACP in POP was significan tly increased compared with that in control group (P<0.001) and had good re lationship with bone density (r=0.82).Conclusions TrA CP was a simple and rapid method for screening and monitoring osteoporosis.