摘要
目的 :研究同种异体肾上腺髓质植入蛛网膜下腔对慢性痛模型大鼠痛行为的影响及其发生机制。方法 :将同种大鼠肾上腺髓质嗜铬细胞异体移植入脊髓背角蛛网膜下腔 ,6w后进行组织学检查 ,并用高压液相色谱法检测脑脊液中嗜铬细胞释放的肾上腺素 (E) ,同时观察移植的嗜铬细胞在 1～ 8w内减轻痛行为的表现。结果 :移植 6w后 ,嗜铬细胞浆内有明显的嗜铬颗粒 ,分泌的肾上腺素明显高于对照组。移植后 1～ 8w内动物的屈关节嘶叫反应评分、关节的肿胀程度、体重的减轻程度 ,移植组均明显低于对照组 (P <0 0 5 )。结论 :移植的嗜铬细胞在蛛网膜下腔能够存活并释放肾上腺素镇痛活性物质 ,减轻慢性痛模型大鼠的痛表现。
Objective:To study the antinociceptive effects of adrenal medullary allografts into the spinal subarachnoid space in chronic pain model rats.Methods:A histological light microscope and a high performance liquid chromatography (HPLC) were used for examining the viability of adrenal medullary allografts 6 weeks after transplantation and at the same time the reduction of chronic pain was observed.Results:Lots of chromaffin cells with many brown granules in tissue section 6 weeks after transplantation were observed.The level of epinephrine in CSF in adrenal implanted group was obviously higher than that in control group.During 1～8 weeks after inoculation,changes of body weights,tibio tarsal joint circumference and vocalization of arthritic rats with adrenal medullary implanted were significantly lower than those of arthritic rats in control group ( P< 0.01).Conclusion:Chromaffin cells allografts in the spinal subarachnoid space survive well,and release neuroactive substance epinephrine to reduce chronic pain.
出处
《山东医科大学学报》
2001年第4期298-300,共3页
Acta Academiae Medicinae Shandong
基金
山东省自然科学基金资助课题 (Y97C0 80 4 4 )
