细胞中酪氨酸酶活性的生化酶学法测定

Biochemical-Enzymic Method on Tyrosinase Activity Determination in Cultured Cells

根据HideyaA .和WrathalJ.介绍的方法 ,建立了细胞中酪氨酸酶活性测定的改进型生化酶学方法 ,以适应美白化妆品研究开发的需要。结果显示 :改进后的方法 ,精确度测试的变异系数均值为 6 2 % ,准确度测试P值 >0 0 5,稳定性测试 ,方差分析无显著性差异。

In order to meet the need of study on skin lightening cosmatic,a inproved method was developed on the base of method introduced by Hideya A and Wrathal J.The results show:the analysis of precision:average CV is 6 2 % ,the analysis of accuracy and stability is not signifricant difference(P>0 05).