枯草芽孢杆菌葡萄糖脱氢酶基因的克隆及其序列分析

Cloning and sequencing of glucose dehydrogenase from B.subtilis

根据Lampel报道的葡萄糖脱氢酶基因序列设计合成两条引物[1] ,以野生型枯草芽孢杆菌染色体DNA为模板 ,PCR扩增得到含有葡萄糖脱氢酶基因的大约 780bp的DNA片段 ,将其克隆到 pUC -T载体中。序列分析表明 ,克隆得到的葡萄糖脱氢酶基因含有 783bp ,编码 2 6 1个氨基酸的蛋白质。得到的基因序列与文献报道的进行比较 ,其核苷酸同源率为 75 .5 % ,编码氨基酸序列的同源率为 83.9%

Chromosomal DNA as template, gene of glucose dehydrogenase from Bacillus subtilis was cloned in plasmid pUC T by PCR. Followed by sequening, the sequence of the fragment from Bacillus subtilis had a little difference from the sequence of glucose dehydrogenase in other paper. Gene of glucose dehydrogenase was composed of 783 base pairs coded for glucose dehydrogenase of 261 amino acids.