苹果发酵液中残余还原糖的测定方法比较

Comparative study on the determination of reducing sugar in cider broth

对传统的费林定糖法、高锰酸钾滴定法和 3,5 -二硝基水杨酸比色定糖法测定苹果原汁及生产酒精的苹果发酵液中的还原糖进行了比较研究。测定结果显示 ,这三种方法的相对误差 ,标准偏差均十分接近。通过对此三种方法的回归相关性分析显示 :比色法 -高锰酸钾法的回归方程为 y =1.0 15 7x + 0 .0 2 6 2 ,相关系数R2 =0 .9996 ;比色法 -费林法的回归方程为 y =0 .973x +0 .2 84 3,相关系数R2 =0 .9984 ;高锰酸钾法 -费林法的回归方程为 y =0 .9914x + 0 .3810 ,R2 =0 .9973。

Assays for reducing sugar in cider broth by using Fehling method, potassium permanganate titration and colorimetric estimation were compared. The results showed that there were similar correlation coefficients among these three methods.However,the colorimetric estimation was easier,quicker and more accurate than the others.