唾液中人类疱疹病毒7型的分离与培养

SURVEY ON HHV-7 ISOLATED AND CULTURED FROM SALIVA

目的 :了解人群中人类疱疹病毒 7型 (HHV- 7)的感染情况 ,并对 HHV- 7进行分离培养、鉴定。方法 :以聚合酶链反应 (PCR)检测唾液中的 HHV- 7,再以巢式 PCR及酶切加以鉴别 ;分离其中 2例标本中的病毒 ,以人脐带血单个核细胞 (HCBMC)进行培养 ,再通过 DNA序列测定来鉴定培养结果。结果 :检测12 0例唾液标本有 97例阳性 ,PCR阳性结果示 186 bp处有一荧光带 ,经 ECORI酶切后成为 12 6 bp和 6 0bp两条荧光带 ,巢式 PCR结果示 94bp处有一荧光带 ,HCBMC可用于 HHV - 7的传代培养。唾液中分离的 HHV - 7与培养结果所测得的病毒序列一致 ,与 Berneman分离测得的 JI株结果相似。结论 :人的唾液中HHV- 7检出率高 ,HHV- 7可在

Objective:To investigate the condition of people infected by human herpesvirus 7(HHV 7),isolate and identify Methods:Saliva sample of people were detected for HHV 7 by nest PCR and were identified by enzyme digest HHV 7 was isolated and cultured,sequenced the 186bp HHV 7 DNA fragment Results:The HHV 7 positive rate was 80 8%(97/120) in the detected inpatients The HHV 7 sequence isolated from saliva was as the same as it isolated from the HCBMC after propagated Conclution:The infection rate is very high in people HHV 7 readily infects and replicates in PHA stimulated HCBMC in vitro