RAPD分析在绢丝昆虫亲缘关系研究中的应用Ⅱ.柞蚕品种间的遗传差异

Application of RAPD Technique in Genetic Relationship of Silk Insect II. Genetic Variance in Antheraea pernyi

采用RAPD技术 ,对 5个柞蚕品种的遗传差异进行比较研究。结果表明 ,所采用的 40个随机引物中 ,有 2 7个引物扩增谱带清晰且重复性较好 ,扩增总片段数 2 5 3条 ,单个引物的扩增片段数在 4～ 16之间 ,片段大小在 0 .33～ 3.0kb之间。不同柞蚕品种间的遗传差异较小 ,遗传距离 (D)在 0 .0 6 6～ 0 .16 5 9之间 ,根据D值 ,由UPGMA聚类分析软件绘制了它们的分子进化树。

Random amplified Polymorphic DNA (RAPD) was used to analyze the genetic diversity among Antheraea pernyi . The genetic variance of five Antheraea pernyi was studied. The result showed that: 27of 40 arbitrary primers could amplify clear and repeating bands. A total of 262 fragments were obtained. Each primer gave 4-16 bands and the average was 9.7. The length of the band was 0.33-3.0kb. The D value between different breeds of Antheraea pernyi was 0.066-0.1659. The D value was used to construct a dendrogram by UPGMA.