褪黑素对免疫性肝纤维化大鼠抗氧化酶活性的影响

Effects of MT on antioxidative enzymes activities in IHF rats induced by human albumine

目的 研究褪黑素对免疫性肝纤维化大鼠血清及肝匀浆中SOD、GSHpx活性的影响。 方法 采用人血清白蛋白诱导大鼠免疫性肝纤维化模型 ,实验大鼠分为正常组、模型组、褪黑素预防组及阳性药对照组 ,检测各组动物血清及肝匀浆中SOD、GSHpx活性。结果 模型大鼠血清及肝匀浆中SOD、GSHpx活性明显降低 ;而自造模的尾静脉攻击阶段起 ,每天ig褪黑素 (0 3、0 6、0 9mg·kg-1) ,均可恢复模型大鼠血清及肝匀浆中SOD、GSHpx的活性。结论 褪黑素对免疫性肝纤维化大鼠模型具有一定程度的保护作用 ,其机制可能与增强其抗氧化酶活性有关。

e Objective To investigate the effects of melatonin(MT) on antioxidative enzymes activities in IHF rats induced by human albumine. Methods The rat immune hepatic fibrosis(IHF) model was induced by human albumine and the activitise of superoxide dismutase(SOD) and glutathione peroxidase(GSHpx) were determined by spectrophotometry. Results MT(0 3、0 6、0 9 mg·kg -1 ) was given daily (ig) for 9 weeks during the attacking stages. The results showed that MT reversed the decreased SOD and GSHpx activities in liver and serum. Conclusion MT has certain effects on the rats with IHF, its mechanisms may be related to the ability of scavenging free radicals.