脾切除对大鼠肝癌形成过程的影响

Effect of splenectomy on the development of liver cancer in rats

目的 探讨脾切除对大鼠实验性诱导的肝癌形成的影响。方法 将SD大鼠随机分成脾切除和模拟脾切除组 ,用二乙基亚硝胺溶液饮喂以诱导SD大鼠肝癌 ,观察肝组织学变化及肝血清变化。结果 脾切除组动物死亡率明显高于对照组 ;脾切除组的肝癌结节数目及大小都有明显增加。肝细胞的异形性及肝癌发生更早、更快 ,NK细胞活性也较对照组明显下降。结论 脾切除对大鼠诱导性肝癌形成发生发展过程有促进作用 ,其发生机制可以与脾脏的基本抗肿瘤免疫功能受损有关。

Objective To investigate the influence of splenectomy on the development of induced liver cancer in rats.Methods Eighty male SD rats (weighting 180 to 220 g) were bred with diethlnitrosarnine (DEN) and then were randomly divided into two groups: total splenectomy group and sham operation group.The changes in histopathology and serum parameters were observed.Results The mortality in the total splenectomy group was significantly higher than that in the control group.The node number of cancer in the total splenectomy group is more than that of control group.In the total splenectomy group, development of hepatic cancer was earlier and quicker and the NK activity significantly lower than in the control group.Conclusion Splenectomy promotes the occurrence and development of induced liver cancer in rat, which might be related with the decline of splenic function of anti tumor immunity.