正交试验法优选从豆制品下脚料中提取大豆皂苷的工艺

Optimization of processes for recovery of soyasaponins from bean product waste by orthogonal design

目的 优选从豆制品下脚料中提取大豆皂苷的最佳提取工艺条件。方法 通过正交试验 ,以紫外分光光度法测定总皂苷含量为指标 ,对提取工艺进行考察。结果 影响回流提取的主要因素为乙醇浓度 ;影响渗漉提取的因素依次为乙醇浓度、溶媒量和渗漉速度。结论 最佳提取工艺条件为 8倍量 60 %乙醇 ,热回流 1次 2 .5 h。

Object To optimize the process for the recovery of soyasaponins from bean product waste. Methods Conditions for the recovery were studied by orthogonal design guided by the yield of soya-saponins as determined by UV absorbance. Results The most influential factor in the recovery by refluxing was the concentration of alcohol; while in the recovery by percolation the influential factors were in the order of concentration of ethyl alcohol, amount of alcohol used and the rate of percolation.Conclusion The optimal recovery process was found to be a single reflux with eight times of 60% alcohol for 2.5 h.