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抗细胞膜DNA自身抗体快速检测系统性红斑狼疮 被引量:7

DNA-associated autoantibodies to cell membrane for rapid detection of systemic lupus erythematosus
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摘要 目的 探讨抗细胞膜DNA自身抗体 (mDNA)在系统性红斑狼疮患者的表达以及这种特异抗体与抗dsDNA抗体的不同。方法 用间接免疫荧光法观察培养的HL6 0细胞的特异性细胞膜的荧光图形。结果 抗细胞膜DNA自身抗体在SLE患者 90例中有 81例阳性 (阳性率 90 % ) ,而在类风湿关节炎 (RA)患者 35例 ,强直性脊柱炎 (AS)患者 31例和健康献血员 42名中均为阴性 ,在干燥综合征 (SS)患者 2 0例中有 1例阳性 (阳性率 5 % )。抗细胞膜DNA自身抗体对SLE诊断的特异性为 98 8%。结论 抗细胞膜DNA自身抗体是一种诊断SLE的新的标记抗体 ,其敏感性高(90 0 % ) ,特异性强 (98 8% ) ,较抗dsDNA抗体、抗Sm抗体检测更先进。 Objective To evaluate the expression of autoantibodies to cell membrane associated with DNA (mDNA) in systemic lupus erythematosus (SLE).The specificity of these antibodies differs from antibodies to double stranded DNA.Method Using indirect immunofluorescence,serum samples were detected giving a characteristic pattern of peripheral membrane fluorescence on cultured HL60.Results This pattern was observed in 81 of 90 serum samplas of SLE patients,but absent in the serum samples of 35 rheumatoid arthritis,31 ankylosing spondylarthritis and 42 blood donors.In 20 Sjgren syndrome′s patients only one showed a positive test.Conclusion This novel rapid immunofluorescence method may serve as an identification test of SLE patients.Due to the sensitivity (90%) and specificity (98 8%),it is better than the other diagnostic tests such as the detection of antibodies to dsDNA and Sm.
出处 《中华风湿病学杂志》 CAS CSCD 2001年第4期227-229,T001,共4页 Chinese Journal of Rheumatology
关键词 系统性红斑狼疮 抗细胞膜自身抗体 荧光免疫测定 抗DSDNA抗体 mDNA 抗SM抗体 Lupus erythematosus,systemic Autoantibodies Antibodies,antinuclear Fluoroimmunoassay
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  • 1Tan E M,Arthritis Rheum,1982年,25卷,1271页

同被引文献36

  • 1苏茵,贾汝琳,栗占国.自身抗体联合检测在系统性红斑狼疮诊断中的意义[J].中华风湿病学杂志,2005,9(1):16-19. 被引量:16
  • 2陈海英,贾汝琳,栗占国.抗细胞膜DNA抗体的测定及其在系统性红斑狼疮诊断中的意义[J].中华风湿病学杂志,2005,9(4):229-233. 被引量:14
  • 3郭嘉隆,毕黎琦,栗占国.抗细胞膜DNA抗体在三种自身抗体阴性系统性红斑狼疮中的诊断价值[J].中华检验医学杂志,2006,29(9):787-790. 被引量:11
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