槲皮素抑制乳腺癌发生及增殖的实验研究

STUDY ON THE INHIBITORY EFFECTS OF QUERCETIN ON THE OCCURRENCE AND PROLIFERATION OF EXPERIMENTAL MAMMARY CARCINOMA

目的 探讨槲皮素抑制乳腺癌发生及增殖的作用。方法 建立二甲基苯丙蒽 (DMBA)诱导的乳腺癌动物模型。 79只雌性SD大鼠随机分为A组 (DMBA)、B组〔DMBA +三苯氧胺 (TAM )〕、C组 (DMBA +槲皮素 )及D组 (空白对照 )。持续喂养 2 8周 ,经光镜、电镜观察 ,抗PCNA及H ras免疫组化分析。结果 ①A组大鼠乳腺肿瘤发生率为76 .2 % ,明显高于B组 ( 4 0 .9% )、C组 ( 4 5.5% )及D组 ( 0 % ) ,P<0 .0 5;B组与C组相比差异无显著性意义 (P>0 .0 5)。②A组大鼠乳腺癌平均直径 2 .37cm ,明显大于B组 ( 1 .82cm)及C组 ( 1 .71cm) ,P<0 .0 5;B组和C组相比差异无显著性意义 (P>0 .0 5)。③PCNA免疫组化染色显示 :A组与B组和C组相比差异有显著性意义 (P<0 .0 5) ,B组和C组相比差异无显著性意义 (P>0 .0 5)。④H ras免疫组化染色显示 :A组与B组和C组比较差异有显著性意义 (P<0 .0 5) ,B组和C组相比差异无显著性意义 (P>0 .0 5)。结论 槲皮素有降低DMBA诱导的SD大鼠乳腺癌发生率及抑制肿瘤生长的作用 ,其机理可能与抑制ras基因活性。

Objective To investigate the inhibitory effects of flavonoids quercetin on the occurrence and proliferation of experimental mammary carcinoma. Methods DMBA induced mammary carcinoma was produced in rats. Seventy nine female Sprague Dawly rats were divided randomly into four groups: DMBA, DMBA with TAM, DMBA with quercetin and control. Chemicals had been administered to group A, group B, group C and group D respectively for 28 weeks. Samples of breasts were collected for light microscope observation and electromicroscope observation. Their expressions of proliferating cell nuclear antigen (PCNA) and the protein product of H ras were examined by immunohistochemical staining. Results ①Mammary carcinoma incidence of group A(76.2%) was significantly higher than that of group B(40.9%), group C(45.5%) and group D(0%), P <0.05, and there was no significant difference between group B and group C ( P >0.05), which indicated that quercetin could inhibit the occurrence of mammary carcinoma. ②Mean mammary tumor diameter of group A ( 2.37 cm) was significantly larger than that of group B (1.82cm) and group C(1.71cm), P < 0.05, and there was no significant difference between group B and group C ( P > 0.05), which indicated that quercetin could inhibit the growth of experimental mammary carcinoma. ③Immunohistochemical staining of PCNA showed significant difference between group A and group B, group A and group C ( P <0.05), with no significant difference between group B and group C ( P >0.05), which indicated that quercetin could inhibit the proliferation rate of tumor cells. ④Significant difference between group A and group B, group A and group C ( P <0.05), and no significant difference between group B and group C ( P >0.05), were noticed with immunohistochemical staining of H ras protein product, which indicated that quercetin could inhibit the activity of H ras protein. Conclusion Quercetin could reduce the mammary carcinoma incidence and its degree of growth, and it may be related with its inhibitory effect on the activity of H ras and the proliferation of tumor cell.