甲状腺病变组织中端粒酶活性的测定及其意义

DETECTION OF TELOMERASE ACTIVITY IN THYROID LESION AND ITS SIGNIFICANCE

目的 了解几种常见甲状腺病变组织中端粒酶活性的区别。方法 用端粒重复序列扩增法测定 1 9例甲状腺癌、1 5例甲状腺癌癌旁组织、2 1例甲状腺腺瘤、1 7例结节性甲状腺肿及 1 3例正常甲状腺组织端粒酶的活性。结果 94.74% ( 1 8/1 9)的甲状腺癌表达出端粒酶活性 ,与癌旁组织 ( 6 .6 7% )、甲状腺腺瘤 ( 4 .6 7% )、结节性甲状腺肿 ( 0 % )及正常甲状腺组织 ( 0 % )比较 ,差异均有显著性意义 (P<0 .0 0 0 1 )。结论 端粒酶是甲状腺癌定性的良好标志物 ,且在甲状腺病变的鉴别诊断中具有重要意义。

Objective To study the difference of telomerase activity in the common thyroid lesions . Methods The telomerase activity was detected in 19 patients with thyroid carcinomas, 15 samples adjacent to thyroid carcinomas,21 specimens of thyromas, 17 cases of nodular goiters and 13 pieces of normal thyroid tissues by telomeric repeat amplification protocol(TRAP). Results Eighteen of 19 samples of thyroid carcinoma, 1 of 15 samples adjacent to the cancer and 1 of 21 adenoma of the thyroid specimens showed positive telomerase activity, all 17 cases of nodular goiters and 18 samples of normal thyroid tissues exhibited negative telomerase activity, and the rate of positive telomerase activity of thyroid carcinomas was significantly higher than that of the other tissues ( P <0.0001). Conclusion The telomerase is an important qualitative marker of thyroid carcinoma and a useful index in differential diagnosis of thyroid lesions.