哌拉西林/舒巴坦体外抗菌活性研究

Study on in vitro Antibacterial Activity of Piperacillin/Sulbactam

目的：研究哌拉西林／舒巴坦（ｐｉｐｅｒａｃｉｌｌｉｎ／ｓｕｌｂａｃｔａｍ）体外抗菌活性。方法：临床分离菌株２６５株，经ＶＩＴＥＫ重新鉴定；采用琼脂平板二倍稀释法测定最低抑菌浓度（ＭＩＣ）；试管二倍稀释法测定最低杀菌浓度（ＭＢＣ）；菌落计数法绘制杀菌曲线；并测定细菌接种量、培养基ｐＨ值以及血清蛋白含量对ＭＩＣ的影响。结果：不同配比哌拉西林／舒巴坦（１：１、２：１、４：１）对临床常见菌的杀菌活性无明显差异，均强于哌拉西林；对Ｇ－杆菌产酶菌抗菌活性显著高于其它对照药，如替卡西林／克拉维酸和氨苄西林／舒巴坦等。结论：哌拉西林／舒巴坦（１：１、２：１、４：１）是一个有效的β－内酰胺酶抑制剂联合制剂，三种配比的哌拉西林／舒巴坦体外抗菌活性相似，有进一步研究开发价值。

OBJECTIVE: The antibacterial activity in vitro of pipercillin/sulbactam were evaluated.METHODS: Piperacillin/sulbactam (l:1,2:1,4:1)against 265 clinical isolated strains was compared with ampicillin/sulbactam, ticarcillin/clavulanic acid and piperacillin. Minimal was determined by use of agar dilution method, Inhibitory bactericidal concentrations (MBC) were measured by tube dilution method. RESULTS: The antibacterial activity of piperacillin/sulbactam(1:1,2:1,4:1) against common clinical isolates was of no difference and better than piperacillin. The antimicrobial activity of piperacillin/sulbactam(1:1,2:1,4:1) against G-bacilli was significantly better than other control antimicrobial agents .The inoculum size,pH and concentration of serum protein did not infuence the in vino activity of piperacillin/sulbactam (1:1,2:1,4:1).CONCLUSION: Piperacillin/sulbactam (1:1,2:1, 4:1) is a effective antibiotic containing β-lactamases inhibitor and valuable to be investigated.