脑膜瘤细胞增殖活性与术后复发的关系

Relationship between Proliferative Activity and Postoperative Recurrence of Meningioma

目的：探讨脑膜瘤的增殖活性与术后复发的关系。方法：采用免疫组化法检测增殖细胞核抗原（PCNA）在98例脑膜瘤（其中复发38例）中的表达，同时进行流式细胞术（FCM）检测，分析脑膜瘤64例（其中复发组34例，非复发组30例）术后复发与DNA含量的关系。结果：脑膜瘤术后复发组与非复发组PCNA标记指数（PCNA LI）分别为11．65±12．49与1．54±2．46，两者比较差异有显著性（P＜0．01），说明脑膜瘤术后复发与PCNA的表达呈显著正相关。同时复发性脑膜瘤的异倍体率（52．3％）、DNA指数DI（1．17±0．12）和增殖指数PI（26．74±10．89）均显著高于非复发组异倍体率（6．7％），DI（1．01±0．11）和PI（14．06±7．68）（P＜0．01）。结论：通过PCNA检测及流式细胞术（FCM）DNA含量分析可较准确地判定肿瘤的增殖潜能，以用于指导临床治疗和判断预后。

Objective To study the relationship between the proliferative activity and postoperative recurrence of meningioma． Methods The expression of PCNA protein was examined in 98 cases by immunohistochemical technique and the content of DNA in 64 cases were detected by flow cytometry FCM． Reasults The PCNA labelling index of recurrence and non－recurrence groups were 11．65±12．49 and 1．54±2．46 respectively with significant difference between these two groups P＜0．01． The heteroploid rate 52．3％ DNA index 1．17±0．12 and proliferative index 26．74±10．89 of recurrent meningiomas were obviously higher than the data in non－recurrent tumors 1．01±0．11 14．06±7．68 and 14．06±7．68 respectively P＜0．01． Conclusion The detection of PCNA protein and analysis of DNA content by FCM could be used to judge the proliferative activity for instructing the clinical therapy and predicting the prognosis of meningioma．