乙型肝炎病毒和黄曲霉素协同在裸鼠体内诱发人胎肝细胞癌变的研究

Study on Malignant Transformation of Human Embryonic Liver Cells Induced by Hepatitis B Virus and Aflatoxin in Nude Mice

为了研究乙型肝炎 (乙肝 )病毒 (HBV)和黄曲霉素 (AFB1)在肝癌发生过程中的作用 ,用HBV感染的人胚胎肝细胞移植至裸鼠背部皮下 ,以后每周注射AFB1,能诱发裸鼠成瘤。实验分为 4组 :A组为HBV +AFB1组 ,即用HBV感染的人胚胎肝细胞移植于裸鼠 ,同时注射ABF1;B组为HBV+ 组 ,用HBV感染的人胚胎肝细胞移植于裸鼠 ,不注射AFB1;C组为AFB1+ 组 ,用不感染HBV的人胚胎肝细胞移植于裸鼠 ,注射AFB1;D组为对照组 ,用不感染HBV的人胚胎肝细胞移植于裸鼠 ,也不注射AFB1。结果 :A组成瘤率为 2 7 3% (6 / 2 2 ) ,B组 0 % ,C组 13 3%(2 / 15 ) ,D组 0 % ,所有肿瘤病理诊断均为肝细胞癌。用EMA单抗检测 ,证实为人来源细胞。PCR和DNA狭缝印迹显示 :HBVX和HBVS基因阳性 ,证明HBV基因已到瘤细胞中。实验首次用人乙肝病毒协同AFB1在裸鼠体内诱发成功人肝细胞癌 ,证明了HBV协同AFB1在人肝细胞癌发生过程中的病因作用。同时 。

In order to study the effect of HBV and aflatoxin in hepatocarcinogenesis,the human embryonic liver cells infected with HBV were transplanted into nude mice by subcutaneous injection The transplanted nude mice were divided into 4 groups Group A(HBV+AFB 1):nude mice were injected with HBV infected human embryonic liver cells,then injected with AFB 1 once a week.Group B(HBV +):nude mice were treated with HBV as Group A ,but no AFB 1 was injected. Group C(AFB + 1):nude mice were injected with normal human embryonic liver cells and treated with AFB 1 as Group A Group D:control group,nude mice were injected with normal human embryonic liver cells and no AFB 1 was used Results:The incidence of tumor in different group was,group A 27 3%(6/22),group B 0% (0/13),group C 13 3%(2/15)and group D 0% (0/14) All the tumors were proved to be human hepatocellular carcinomas(HCCs)by pathological diagnosis The tumor tissues were anthropogenetic tested by EMA monoclonal antibody The HBV-X and HBV-S genes were positive by slot hybridization and PCR amplification,indicating that HBV DNA genes had integrated into cellular DNA We have first successfully induced human HCC with HBV and AFB 1 in nude mice This study demonstrated that there is a synergetic effect between HBV and AFB 1 in human hepatocarcinogenesis and will offer an animal model for further research on liver cancer