摘要
To investigate the HBV quasispecies population in the patients with chronic HBV infection,a set of specific primers was synthesized according to DNA sequence of HBV found in China The reverse transcriptase(RT) region in polymerase gene was amplified by PCR method from the sera of 3 patients with chronic HBV infection,and then the PCR products were subcloned into pGEM Teasy vectors 13 clones were sequenced Sequence comparison of the selected clones was made to find out the differences After being compared,11 sequences of RT were found different The different rates of RT coding nucletide sequences, RT and HBsAg amino acid sequences of the 11 clones were 5 1%,4 9% and 7 5% respectively Many mutation types,including point substitution and deletion mutation,were found in this region There are quasispecies population and defective HBV genome in patients with chronic HBV infection.
To investigate the HBV quasispecies population in the patients with chronic HBV infection,a set of specific primers was synthesized according to DNA sequence of HBV found in China The reverse transcriptase(RT) region in polymerase gene was amplified by PCR method from the sera of 3 patients with chronic HBV infection,and then the PCR products were subcloned into pGEM Teasy vectors 13 clones were sequenced Sequence comparison of the selected clones was made to find out the differences After being compared,11 sequences of RT were found different The different rates of RT coding nucletide sequences, RT and HBsAg amino acid sequences of the 11 clones were 5 1%,4 9% and 7 5% respectively Many mutation types,including point substitution and deletion mutation,were found in this region There are quasispecies population and defective HBV genome in patients with chronic HBV infection.
出处
《病毒学报》
CAS
CSCD
北大核心
2001年第3期270-272,共3页
Chinese Journal of Virology
关键词
乙型肝炎病毒
准种
变异
多聚酶
逆转录酶区
hepatitis B virus
quasispecies
mutation
reverse transcriptase region
