螺旋藻批式与连续培养及其生长动力学

Studies on the Growth Characteristics of Spirulina Cells Under Batch and Continuous Conditions

在内循环气升式光生物反应器中 ,分别研究了螺旋藻细胞在批式和连续培养条件下的生长特性 ,结果表明 :Richards模型和指数衰减模型可较好地描述批式培养时细胞和碳源底物浓度与培养时间的关系 ;批式培养时最大细胞生长速率为 0 371g d L ,细胞对碳的得率系数为 3 439g gC ;连续培养时随着稀释率的增大 ,细胞和底物浓度分别呈下降和上升趋势 ;连续培养时最大细胞产率为 0 36 2g L d ,最佳稀释率为 0 45 d ,细胞对碳的得率系数为2 0 5 0g gC ;所提出的连续培养动力学模型可较好地拟合实验数据。

The research paper focused mainly on the growth characteristics of \%Spirulina\% cells under batch and continuous conditions.It was found that the Richards and Exponential decay model could well express the relationships between the concentration of the cell,carbon in the medium and culture time respectively.The maximum cell growth rate and the cell yield coefficient against carbon under batch culture was 0.371g/d/L and 3.439g/gC respectively.The concentration of cell and that of carbon in the reactor increased and decreased with the dilution rate respectively under the continuous culture condition.The maximum cell growth rate and the optimum dilution rate was 0.362g/d/L and 0.45/d respectivel.The cell yield against carbon under continuous culture condition was 2.050g/gC.The cell growth rate of batch culture was larger than that of continuous one when the cell concentration was relatively low,but it will be smaller than that of continuous culture when the cell concentration became high.