真菌诱导子对悬浮培养南方红豆杉细胞态势及紫杉醇合成的影响

Effects of Fungal Elicitor on Cell Status and Taxol Production in Cells Suspension Cultures of Taxus chinesis var.mairei

在南方红豆杉细胞悬浮培养过程中 ,研究了在指数生长期的末期加入真菌诱导子 (尖孢镰刀菌的胞壁组分粗提物 )对细胞态势及紫杉醇合成的影响。结果表明 ,真菌诱导子能在短期内激发细胞的防御性应答反应而产生氧迸发 ,细胞的氧化还原态势发生了明显的变化 ,培养基发生碱化现象 ,表征酶含量的蛋白质含量明显提高 ,SOD、POD、CAT的活力出现波动性变化 ,并具有一定的时序性。同时 ,南方红豆杉悬浮培养体系中产次生代谢途径中重要的酶PAL的活力得到提高 ,紫杉醇的合成被加强 ,产量得到了显著提高 ,达到了对照组的 5倍左右。

Effects of fungal elicitor on cell redox status and taxol production were studied in suspension cultures of \%Taxus chinesis\% var.\%mairei\% in the late exponential stage.The results show that fungal elicitor induced oxygen burst,changes of the cell redox status,alkalinization of medium and the fluctuation of the activity of redox enzymes with a sequence.The content of protein representing the quantity of enzymes increased.The activity of SOD increased quickly after treatment by fungal elicitor and that of POD could be kept at a higher level in contrast to the control.The activity of CAT was inhibited at first and followed by an obvious increase,while the activity of PAL was promoted.The taxol yeild was 5 folds of the control,reaching to 67mg/L.