他莫昔酚对耐药细胞系K562/DOX多药耐药逆转作用的研究

Study on the reversing effect of tamoxifen on multidrug resistant cell line K562/DOX

目的：寻找有效的多药耐药逆转剂。方法：以他莫昔酚作为耐药逆转剂，用［溴化－３（４，５－二甲基－２－噻唑基）－２，５－二苯基四唑氮］ＭＴＴ法体外药物敏感试验，观察他莫昔酚对多药耐药白血病细胞株Ｋ５６２／ＤＯＸ的药物敏感性的影响。结果：他莫昔酚可增强阿霉素（ＤＯＸ）对Ｋ５６２／ＤＯＸ的细胞毒作用，对Ｋ５６２细胞无影响。结论：他莫昔酚是一种有效的多药耐药逆转剂。

OBJECTIVE:To develop effect of tamoxifen on resistance modulators. METHODS: The effects of tamoxifen on drug sensitivity in multidrug resistant human leukemic cell line K562/DOX were studied by MTT corlori- metric assay. RESULTS:Tamoxifen enhanced the cytotoxicity of doxorubicin (DOX) to K562/DOX and had no effect on efflux of DOX in K562 cells. CONCLUSION: Tamoxifen is an effective reversal agent of multidrug resistance.