HPLC法测定华海乙肝泡腾颗粒剂中柴胡皂苷C的含量

Determination of saikosaponin C in Huahai-yigan granules by HPLC

目的：为控制华海乙肝泡腾颗粒剂的质量建立测定柴胡皂苷Ｃ的方法。方法：采用高效液相色谱法，色谱条件为：ＯＤＳ柱，以甲醇－水（６１：３９）为流动相，流这 １．０ｍｌ／ｍｉｎ，检测波长２０３ｎｍ。结果：平均回收率９９．３％，ＲＳＤ＝１．３４％。结论：此法可作为控制华海乙肝泡腾颗粒剂质量的方法之一。

OBJECTIVE: To determine saikosaponin C in Huahai-yigan granules. METKODS:A chromatography method of HPLC for the determination wan eat8blished an ODS column with methanol-water( 61 :39) as the me bile phase at 1.0ml/min flow-rate, detection wavelength at 203 nm. RESULTS: The average recovery was 99. 3% and RSD was 1.34%. CONCLUSIONS :This method is available fur quality control of Huahai-yigan granules.